Fig. 2.

Restoration of glucose uptake upon IL-7 readdition is dependent on phosphorylation of the hexose. IL-7-dependent D1 T cells were incubated without cytokine for 18 h and IL-7 (50 ng/ml) readded at the time points specified in the figures. Controls included were D1 T cells grown continuously in IL-7 (+IL-7) and D1 T cells deprived of IL-7 for 18 h (−IL-7). D1 T cells were assayed for glucose use with 2-DOG (A) or glucose import with 3-OMG (B) as described in materials and methods. Results are representative of 3 independent experiments performed in triplicate (values are average ± SD). *P < 0.05 compared with cells cultured −IL-7.