Fig. 3.

Hypoxic stress causes rapid, transient phosphorylation of MLC. A: we used confocal microscopy (×63 objective) to assess MLC phosphorylation over the time course of hypoxic stress. WGA-TR, wheat germ agglutinin-tetramethylrhodamine. B: hypoxia caused a dramatic and significant increase in phosphorylated MLC (pMLC) within 15 min (***P < 0.001) that was quickly restored to basal levels. Levels of pMLC remained similar to control (normoxic) for the duration of the hypoxic stress (6 h). Data are presented as means ± SE for 3 confocal stacks.