Table 1.

Expression of proteins involved in cytoskeletal contraction and/or actin remodeling following hypoxic stress

Protein	Accession No.	Average Expression (1 h)	Average Expression (3 h)	Average Expression (6 h)
α1-Actin	P62737	1.45 ± 0.11†	0.74 ± 0.03*	0.93 ± 0.04
α1-Actinin	Q7TPR4	1.25 ± 0.28	0.89 ± 0.05	1.25 ± 0.28
α4-Actinin	P57780	0.90 ± 0.04	0.90 ± 0.09	0.90 ± 0.03
ARP3	Q99JY9	1.33 ± 0.34	1.09 ± 0.36	1.03 ± 0.35
Cofilin-1	P168760	0.95 ± 0.03	0.99 ± 0.07	0.93 ± 0.05
Moesin	P26041	0.98 ± 0.03	1.01 ± 0.03	0.99 ± 0.04
MHC9	Q8VDD5	1.08 ± 0.27	0.94 ± 0.13	0.94 ± 0.17
MHC10	Q61879	0.97 ± 0.34	0.79 ± 0.08	0.84 ± 0.18
MLC6	Q60605	1.10 ± 0.26	0.94 ± 0.16	0.95 ± 0.13
MLC9	Q9CQ19	1.85 ± 0.66		1.30 ± 0.36
Paxillin	Q8VI36	0.97 ± 0.03	0.77 ± 0.05	0.94 ± 0.29
Profilin-1	P62962	1.17 ± 0.23	1.20 ± 0.16	1.11 ± 0.20
RhoA	Q9QUI0	0.89 ± 0.07	0.88 ± 0.05	0.88 ± 0.07
ROCK	P70336	0.88 ± 0.04	0.82 ± 0.16	0.84 ± 0.14
α-Spectrin	P16546	1.14 ± 0.13	1.12 ± 0.08	1.40 ± 0.31
β-Spectrin	Q62261	0.92 ± 0.08	0.95 ± 0.05	1.05 ± 0.11
Talin-1	P26039	0.94 ± 0.08	0.93 ± 0.07	0.94 ± 0.07
Thymosin-β4	P20065	1.48 ± 0.49	1.42 ± 0.37	1.14 ± 0.22
Tropomyosin 4	Q6IRU2	0.94 ± 0.06	0.98 ± 0.03	0.98 ± 0.02
VASP	P70460	1.12 ± 0.23	0.96 ± 0.09	1.17 ± 0.11
Vinculin	Q64727	0.98 ± 0.01	0.98 ± 0.02	0.95 ± 0.004
Zyxin	Q62523	0.87 ± 0.07	0.86 ± 0.14	0.87 ± 0.10

Data are means ± SE for 3 separate labeling experiments. Protein extracts were labeled with isobaric tags (iTRAQ system), and relative expression (normalized to control expression) was determined. ARP3, actin-related protein 3; MHC, myosin heavy chain; MLC, myosin light chain; RhoA, Ras homolog gene family A; ROCK, Rho kinase; VASP, vasodilator-stimulated phosphoprotein.

^*P < 0.05,

^†P < 0.001 vs. control.