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. 2010 Mar 16;18(6):1155–1164. doi: 10.1038/mt.2010.43

Figure 4.

Figure 4

High-resolution analysis of heterofusion between green MM1 and red KAS 6/1 cells which resulted in observation of dual colored syncytia. At 18 hours postinfection, MV-Luc infected (MOI 1.0) MM1 cells (labeled green with CellTracker Green CMFDA) were mixed with KAS 6/1 target cells (labeled red with CellTracker Red CMPTX) at a 1:1 ratio. At (a) 2 hours, (b) 48 hours, and (c) 72 hours after mixing, the cells were photographed using a fluorescence microscope at (original magnification ×200) under blue and green light separately and the photographs were merged, yielding “orange colored” syncytia. (d) Coculture of uninfected MM1 cells (green) with KAS 6/1 cells (red) did not result in heterofusion of cells or syncytia. Bars = 50 µm. MM, multiple myeloma; MOI, multiplicities of infection; MV, measles virus.