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. 2010 Apr 13;18(6):1103–1110. doi: 10.1038/mt.2010.57

Figure 1.

Figure 1

Construction of targeting vector and screening of ES clones. (a) Schematic of targeting vector and screening strategy. Correct knock-in of the GFP* reporter cassette to the ROSA26 locus causes the addition of an upstream EcoRV site resulting in a 2.2 kb fragment upon digestion. (b) Southern blot analysis of correct knock-in ES clones. Genomic DNA was purified from ES clones, digested with EcoRV and used for Southern analysis. ES, embryonic stem; kb, kilobase; PGK-DTA, diphtheria toxin cassette; PGK-Neo, neomycin resistance cassette; SA, splice acceptor.