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Teratoma formation assay. Two teratomas resulting from subcutaneous injection of targeted cells were harvested from nude mice. (a) Teratomas were initiated by gene-targeted ES cells as demonstrated by GFP fluorescence. Teratomas were also photographed under a Cy3 filter to show background fluorescence. (b) Sections were cut and stained with hematoxylin and eosin to identify tissue structures indicated in the figure. n, neuroepithelial cells; o, osteoid tissue; e, epithelium.
