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. 2010 Mar 31;59(7):1648–1656. doi: 10.2337/db09-0287

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© 2010 by the American Diabetes Association.

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FIG. 1. — Immunohistochemistry of subcutaneous adipose tissue in obesity. A: Formalin-fixed adipose tissue section stained for CD68 showing a crown (*) and several resident ATMs (arrowheads). B: Serial section stained for CD11c showing crown ATMs preferentially expressing CD11c. C: Formalin-fixed adipose section stained for CD11c and CD68 or with nonspecific isotype control antibodies confirming that crown ATMs (upper panel) but not resident ATMs (middle panel) express CD11c. D: Frozen adipose tissue section stained for CD206 showing a crown (*) and several resident ATMs. E: Frozen section stained for CD206 and CD11c (upper panel) or with isotype control antibodies (lower panel) showing crown ATMs expressing both CD11c and CD206 but resident ATMs expressing only CD206. Scale bar in all images = 50 μm. (A high-quality digital representation of this figure is available in the online issue.)