FIG. 4.

NCX1 inhibition and Ca²⁺-dependent exocytosis in β- and α-cells. Representative capacitance measurements and grouped data of cumulative changes at individual pulse numbers from mouse and human β-cells with reduced NCX1 expression (Ad-shNCX1), scrambled control vector (Ad-scramble) (A), or in the presence of 1μmol/l KB-R7943 (C and E) in response to a depolarizing train of membrane potential pulses. B: Western blot analysis (i, ii) showing that NCX1 protein expression is only partially reduced as a result of Ad-shNCX1 infection in MIN6 cells (i–iii). β-actin (i) and total ACC (ii) were used as loading controls. D: Representative capacitance measurements and grouped data for calcium infusion experiments in the presence and absence of 1μmol/l KB-R7943. F: Representative capacitance measurements and grouped data from mouse α-cells in response to 1 μmol/l KB-R7943. *P < 0.05, n = 8–20 cells per group or three separate lysates (B).