FIG. 1.
Functional detection of endogenous GABAAR Cl− channels in human islet cells. A: Patch-clamp recording of currents evoked by puffer application of GABA (1 mmol/l, as indicated by the bars) to an identified β-cell in the absence (black trace) and presence (gray trace) of 50 μmol/l SR-95531 in the same cell. The cell was held at −70 mV throughout the experiment. B: As in A, showing a δ-cell (note the difference in scale bars). C: As in A, showing an α-cell. The cell had been incubated in the presence of 0.5 μmol/l insulin for 1 h before the experiment.