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. 2010 Apr 22;59(7):1702–1708. doi: 10.2337/db09-1698

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© 2010 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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FIG. 1. — Imunohistochemical double staining for replication markers and pancreatic cell type–specific markers in paraffin sections of human donor pancreas. Donors with a relatively high level of β-cell replication (≥90th percentile) (high) show increased replication in endocrine and ductal cell types compared with donors with a low level of β-cell replication (<90th percentile) (low). Representative images are shown. Staining for Ki67 (brown) and insulin (red) shows replicating cells in and around islets of Langerhans (A and B; ×70); part of the replicating islet cells correspond to insulin-positive β-cells (C and D; blue arrows; ×200). Staining for the G₂ to M transition marker phosphohistone H3 (brown) and insulin (red) shows an immunopositive mitotic β-cell (E and F; blue arrow; ×200). Staining for the transcription factor Nkx6.1 (brown) and insulin (red) shows colocalization of the two markers (G and H; ×200). Staining for Ki67 (brown) and glucagon (red) shows replicating α-cells (I and J; blue arrows; ×200). Double staining for Ki67 (brown) and somatostatin (red) shows replicating δ-cells (K and L; blue arrows; ×200). Double staining for Ki67 (brown) and the panendocrine marker synaptophysin (red) shows replicating endocrine islet cells (M and N; ×200). Double labeling for Ki67 (brown) and the ductal marker CA19.9 (red) shows replicating ductal cells (O and P; blue arrow; ×200). (A high-quality digital representation of this figure is available in the online issue.)