FIG. 2.

Effects of sDPP-IV and incretins on the migration of CD4⁺ T-cells. For these and all subsequent studies, CD4⁺ T-cells were isolated from nondiabetic female NOD mice receiving NCD. Responses of CD4⁺ T-cells from the spleen (A), thymus (B), and lymph nodes (LNs) (C) stimulated with sDPP-IV (100 mU/ml), GIP, or GLP-1 (100 nmol/l) in the presence or absence of the DPP-IV inhibitor sitagliptin (100 μmol/l) for 1 h. “Control” migration is CD4⁺ T-cell migration in the absence of sDPP-IV, GIP, or GLP-1. To further delineate the subset of responsive lymph node CD4⁺ T-cells, cells were isolated from the mesenteric (D), inguinal (E), cervical (F), and auxiliary (G) lymph nodes and treated in an identical fashion to those in (A–C). All data are means ± SEM, and significance was tested using ANOVA with a Newman-Keuls post hoc test. **P < 0.05 vs. control.