FIG. 5.

CTGF increases miR-192/215 expression. A: NRK52E cells were infected with Ad-CTGF or Ad-vector, and miR-192/215 levels were assessed 6 days after infection (4). At this time point, ECM production and E-cadherin RNA levels are increased as previously reported (4). miR-192/215 levels were significantly increased by CTGF (*P < 0.01 and *P < 0.05, respectively, compared with empty vector control). B: NRK52E cells were treated with rhCTGF (250 ng/ml) for 6 days (4), and miR-192/215 levels were assessed and were both found to be significantly increased (*P < 0.05) compared with control. C: Schema showing the mechanism by which the profibrotic factors TGF-β and CTGF regulate E-cadherin expression and ECM accumulation. However, they also regulate the expression of miR-192/215 in opposite directions and, consequently, the expression of ZEB2, the transcriptional regulator of E-cadherin, and E-cadherin. Error bars represent ± SEM.