Table 2.
TaALMT1 expression and malate efflux from T2 lines
| Wheat lines | TaALMT1 expression (relative to BW26) | Malate efflux (nmol apex−1 h−1) |
|---|---|---|
| Controls | ||
| BW26 | 1·0 | 0·4 ± 0·1 |
| ET8 | 6·7 | 1·7 ± 0·2 |
| T2_1-19 (null) | 0·7 | 0·5 ± 0·1 |
| T2 lines | ||
| T2_1·6 | 5·0 | 2·1 ± 0·2 |
| T2_2B.1 | 2·3 | 2·2 ± 0·2 |
| T2_3·4 | 1·7 | 1·6 ± 0·1 |
| T2_4·4 | 1·8 | 2·0 ± 0·4 |
| T2_5·4 | 3·1 | 1·6 ± 0·1 |
| T2_8·5 | 0·8 | 1·2 ± 0·1 |
| T2_12·7 | 4·5 | 1·7 ± 0·2 |
| T2_18·1 | 5·1 | 2·1 ± 0·1 |
| T2_20A.8 | 5·9 | 1·7 ± 0·2 |
Expression in the root apices was estimated by qRT–PCR using the GAPDH gene as an internal reference. The values shown are expressed relative to the expression in BW26 which was included in each experiment. Data are the mean of three technical replicates. Malate efflux was measured from excised root apices in the presence of 50 µm AlCl3. Data show the mean and standard error (n = 4).