Table 6. Functions predicted for proteins encoded in the transcriptome of the adult stage of each Clonorchis sinensis and Opisthorchis viverrini based on gene ontology (GO).
| Parental GO terms | C. sinensis sequences (%)a | O. viverrini sequences (%) | Top GO term for C. sinensis (CS) and O. viverrini (OV) |
| Biological process GO:0008150 | |||
| Anatomical structure formation GO:0010926 | 134 (1.26) | 124 (1.02) | Protein polymerization GO:0051258 (CS:56; OV:37)b |
| Biological adhesion GO:0022610 | 76 (0.72) | 100 (0.83) | Homophilic cell adhesion GO:0007156 (CS:44; OV:65) |
| Biological regulation GO:0065007 | 829 (7.82) | 952 (7.86) | Regulation of transcription, DNA-dependent GO:0006355 (CS:138;OV:163) |
| Cellular component biogenesis GO:0044085 | 164 (1.55) | 155 (1.28) | Protein polymerization GO:0051258 (CS:56; OV:37) |
| Cellular component organization GO:0016043 | 243 (2.29) | 247 (2.04) | Protein polymerization GO:0051258 (CS:56; OV:37) |
| Cellular process GO:0009987 | 3786 (35.70) | 4254 (35.13) | Protein amino acid phosphorylation GO:0006468 (CS:321; OV:345) |
| Death GO:0016265 | 10 (0.09) | 17 (0.14) | Regulation of apoptosis GO:0042981 (CS:6; OV:11) |
| Developmental process GO:0032502 | 27 (0.25) | 40 (0.33) | Multicellular organismal development GO:0007275 (CS:18; OV:21) |
| Growth GO:0040007 | 1 (0.01) | 1 (0.01) | Regulation of cell growth GO:0001558 (CS:1; OV:1) |
| Immune system process GO:0002376 | 4 (0.04) | 3 (0.02) | Immune response GO:0006955 (CS:3; OV:2) |
| Localization GO:0051179 | 852 (8.03) | 994 (8.21) | Transport GO:0006810 (CS:204; OV:236) |
| Locomotion GO:0040011 | 2 (0.02) | 3 (0.02) | Ciliary or flagellar motility GO:0001539 (CS:1; OV:2) |
| Metabolic process GO:0008152 | 3516 (33.15) | 4113 (33.96) | Protein amino acid phosphorylation GO:0006468 (CS:321; OV:345) |
| Multicellular organismal process GO:0032501 | 28 (0.26) | 37 (0.31) | Multicellular organismal development GO:0007275 (CS:18; OV:21) |
| Multi-organism process GO:0051704 | 1 (0.01) | 1 (0.01) | Pathogenesis GO:0009405 (CS:1; OV:1) |
| Regulation of biological process GO:0050789 | 805 (7.59) | 920 (7.60) | Regulation of transcription, DNA-dependent GO:0006355 (CS:138; OV:163) |
| Reproduction GO:0000003 | 3 (0.03) | 8 (0.07) | Spermatogenesis GO:0007283 (CS:2; OV:1) |
| Response to stimulus GO:0050896 | 123 (1.16) | 140 (1.16) | DNA repair GO:0006281 (CS:49; OV:51) |
| Viral reproduction GO:0016032 | 1 (0.01) | 1 (0.01) | Viral genome replication GO:0019079 (CS:1), viral transcription GO:0019083 (OV:1) |
| Cellular component GO:0005575 | |||
| Cell GO:0005623 | 2953 (60.19) | 3393 (61.55) | Intracellular GO:0005622 (CS:640; OV:707), |
| Envelope GO:0031975 | 59 (1.20) | 66 (1.20) | Nuclear pore GO:0005643 (CS:18; OV:18) |
| Extracellular region GO:0005576 | 85 (1.73) | 110 (2.00) | Proteinaceous extracellular matrix GO:0005578 (CS:12; OV:13) |
| Macromolecular complex GO:0032991 | 671 (13.68) | 705 (12.79) | Ribosome GO:0005840 (CS:128; OV:141) |
| Membrane-enclosed lumen GO:0031974 | 58 (1.18) | 59 (1.07) | Mediator complex GO:0000119 (CS:14; OV:14) |
| Organelle GO:0043226 | 1057 (21.55) | 1160 (21.04) | Nucleus GO:0005634 (CS:368; OV:413) |
| Synapse GO:0045202 | 22 (0.45) | 19 (0.34) | Postsynaptic membrane GO:0045211 (CS:20; OV:17) |
| Virion GO:0019012 | 1 (0.02) | 1 (0.02) | Viral capsid GO:0019028 (CS:1) viral nucleocapsid GO:0019013 (OV:1) |
| Molecular function GO:0003674 | |||
| Antioxidant activity GO:0016209 | 10 (0.09) | 16 (0.13) | Glutathione peroxidase activity GO:0004602 (CS:4; OV:6) |
| Auxiliary transport protein activity GO:0015457 | 1 (0.01) | Sodium channel inhibitor activity GO:0019871 (OV:1) | |
| Binding GO:0005488 | 5160 (48.56) | 5757 (47.81) | ATP binding GO:0005524 (CS:919; OV:1012) |
| Catalytic activity GO:0003824 | 4159 (39.14) | 4733 (39.30) | Protein kinase activity GO:0004672 (CS:289; OV:316) |
| Electron carrier activity GO:0009055 | 68 (0.64) | 96 (0.80) | Electron carrier activity GO:0009055 (CS:68; OV:96) |
| Enzyme regulator activity GO:0030234 | 180 (1.69) | 221 (1.84) | Serine-type endopeptidase inhibitor activity GO:0004867 (CS:33; OV:67) |
| Metallochaperone activity GO:0016530 | 1 (0.01) | Copper chaperone activity GO:0016531 (CS:1) | |
| Molecular transducer activity GO:0060089 | 111 (1.04) | 124 (1.03) | Signal transducer activity GO:0004871 (CS:38; OV:49) |
| Nutrient reservoir activity GO:0045735 | 2 (0.02) | 2 (0.02) | Nutrient reservoir activity GO:0045735 (CS:2; OV:2) |
| Proteasome regulator activity GO:0010860 | 2 (0.02) | 1 (0.01) | Proteasome activator activity GO:0008538 (CS:2; OV:1) |
| Structural molecule activity GO:0005198 | 218 (2.05) | 224 (1.86) | Structural constituent of ribosome GO:0003735 (CS:132; OV:145) |
| Transcription regulator activity GO:0030528 | 197 (1.85) | 229 (1.90) | Transcription factor activity GO:0003700 (CS:99; OV:121) |
| Translation regulator activity GO:0045182 | 29 (0.27) | 31 (0.26) | Translation initiation factor activity GO:0003743 (CS:17, OV:22) |
| Transporter activity GO:0005215 | 489 (4.60) | 607 (5.04) | Atpase activity, coupled to transmembrane movement of ions, phosphorylative mechanism GO:0015662 (CS:48; OV:63) |
a
Values in parentheses are the percentage of the total number of predicted proteins within each GO category (i.e. biological process, molecular function or cellular component).
b
The most frequently reported GO category and number of sequences within each category are summarized for each parental GO category.
The parental (i.e. level 2) and specific GO categories were assigned according to InterPro domains with homology to functionally annotated genes.