Figure 5. GFP and cellular markers sca-1 and c-kit co-localize in AAV-HIMF-treated bone marrow transplant recipients.

Frozen lung sections from bone marrow transplant recipients treated with AAV-HIMF (2.5×10¹⁰ VP, 14d) were stained with antibodies for (B) sca-1, (F) c-kit, or (J) CD34 (red). (C, G, K) GFP signal was obtained through direct visualization (green). (A, E, I) Cell nuclei were counterstained with DAPI (blue). (D, H, L) The arrows in the merged images demonstrate co-localization of GFP with the cellular markers. Scale bar: 20 µm.