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. 2010 Jun 22;5(6):e11253. doi: 10.1371/journal.pone.0011253

Figure 4. FeNG induces apoptosis in CEM/ADR5000 cell line.

Figure 4

(A)CEM/ADR5000 cells were incubated with FeNG for the indicated time and then stained with annexin- FITC, which specifically detects exposed phosphatidyl serine residues at the cell surface. The number of annexin-V-positive cells was determined using a flow cytometer and the percentage is represented in each panel. (B) Cell cycle distribution of CEM/ADR5000 cells after FeNG treatement. CEM/ADR5000 cells treated with FeNG alone or in combination with 5 mM NAC for indicated times were harvested and fixed in 70% ethanol. After staining with propidium iodide they were analyzed using a flow cytometer. The percentage of cells in the sub-G1 (representative of hypodiploid DNA content) population is indicated in each panel.