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. 2010 Jun 22;5(6):e11263. doi: 10.1371/journal.pone.0011263

Figure 1. ‘Cut and Paste’ approach used to construct an orthogonal M. jannaschii tRNA synthetase-tRNA pair for use in mammalian cells.

Figure 1

(A) The M. jannaschii tRNA synthetase (RS) CP1 region (red) was replaced with the E. coli RS CP1 region (blue) to create mutant M. jannaschii RS CP1. (B) The mutations in the M. jannaschii tyrosyl tRNA mutant are shown in red.