Figure 5. Deadenylase activity of Nocturnin was not involved in miR-122-mediated self-regulation.

A. Relative luciferase activities (means ± S.E. Three independent experiments in duplicate) with miR-122 overexpression in MEFs (White bars; +/+, Gray bars; +/−, Black bars; −/−). Luciferase activities were normalized as described in Figure 2. Asterisks represent p<0.005 versus no miR-122. B. miR-122 expression was measured from Noc ^+/+ and Noc ^−/− livers. RNA samples are pools of equal amounts of RNA from each circadian time point (ZT 0, 4, 8, 12, 16, and 20, one mouse per time point).