Table 1. Relative number of infectious virus particles added to different cell lines to achieve MOI 3 for each virus/cell combination.
VSV | SeV | |
BHK21 | 3.0 CIUBHK/cell | 7.5 CIUHeLa/cell |
BSRT7 | 22.5 CIUBHK/cell | 3.2 CIUHeLa/cell |
HeLa | 132.0 CIUBHK/cell | 3.0 CIUHeLa/cell |
A549 | 13.2 CIUBHK/cell | 8.1 CIUHeLa/cell |
4T1 | 227.0 CIUBHK/cell | 37.8 CIUHeLa/cell |
HEp2 | 227.0 CIUBHK/cell | 4.8 CIUHeLa/cell |
Vero | 23.7 CIUBHK/cell | 4.3 CIUHeLa/cell |
Most experiments in this study were conducted using 24-well tissue culture plates and nearly 100% confluent cells treated with RBV in SFM (or mock-treated with SFM) and infected with VSV-GFP or SeV-GFP (or mock-infected with SFM) at MOI of 3 CIU/cell of the tested cell line. The MOI of 3 CIU/cell for each virus/cell type combination was calculated by infecting each cell line with serial dilutions of VSV-GFP or SeV-GFP virus stock in SFM and counting infectious foci with the aid of fluorescence microscopy (see Figure 7A). VSV CIUBHK – number of cell infectious units (infectious particles) determined by titration of VSV-GFP virus stock on BHK21 cells. SeV CIUHeLa - number of cell infectious units calculated by titration of SeV-GFP virus stock on HeLa cells.