Table 2.
Jurkat T-cells were treated with 10 nM NAI, 10 μM JNK-I or10 nM PKC-I for 2 h followed by induction with 162 nM PMA for 24 h.
C | PMA | NAI | JNK-I | PKC-I | |
---|---|---|---|---|---|
IL-6 | 11.7 ± 2.4 | 63.6 ± 11.9 | 3.3 ± 4.0* | 2.7 ± 2.2** | 39.0 ± 20.8 |
CXCL8 | 450 ± 139 | 50294 ± 19161 | 7411 ± 545** | 3197 ± 1079* | 23238 ± 2547 |
Gene expression was determined using RT-qPCR. The Ct values were normalized against 18S. Statistical significance from the positive control (PMA) was determined using Student's t-test. Concentrations are given as fg/ml, values are presented as mean ± SEM, n = 3.