Figure 5.

RNAi against XPA sensitizes colon cancer cells to cisplatin. HCT116, HCT116 + chr3 and HCT116p53-/- cells were transfected with non-targeting control (NT) or XPA siRNA. These transfected cells were exposed to the indicated concentration of cisplatin and apoptosis was assessed 48 hours later by the proportion of cells with subdiploid DNA content. Each value represents the mean (± SEM) determined from a minimum of 3 independent experiments. An * indicates that the value is significantly different its respective NT control (P ≤ 0.05, t-test). Similar results were obtained following exposure to UV light (data not shown).