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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Cancer. 2010 Jun 1;116(11 Suppl):2806–2814. doi: 10.1002/cncr.25167

Figure 1. Cox-2 mRNA, Protein And EP Receptor Protein Production By Breast Cancer Cells.

Figure 1

Figure 1A. Cox-2 Gene Expression. The SUM190 and SUM149 IBC tumor cells expressed Cox-2 at high levels compared to Cox-2 mRNA levels expressed by MCF-7 and MDA-MB-231 breast cancer cells.

Figure 1B. Western Blot Of Cox-2, EP3 And EP4 Receptors. SUM149 and SUM190 IBC cells produce high levels of Cox-2 protein. All cell lines produce both EP3 and EP4 receptor proteins, with greater levels of EP4 proteins produced by SUM190 and SUM149 cells compared to the MCF-7 and MDA-MB-231 breast tumor cells.

Figure 1C. PGE2 Production. Both SUM190 and SUM149 cells produced significantly greater levels of endogenous PGE2 (p<0.05) that either MCF-7 or MDA-MB-231 breast cancer cells. Addition of arachidonic acid significantly increase PGE2 production by SUM190 and SUM149 IBC cells.