Figure 2A. Effect of PGE2 and EP4 Agonist PGE1-Alcohol on Invasion. PGE2 and the EP4 agonist PGE1-alcohol induced significantly increased invasion of SUM149 cells but had no effect on invasion of MDA-MB-231 cells.
Figure 2B. Effect Of GW627368X On Invasion. GW627368X, induced a significant dose dependent inhibition of invasion beginning at the lowest concentration of 0.1 μM, compared to the inhibition of invasion by MDA-MB-231 by GW627368X at higher concentrations.
Figure 2C. Effect Of EP4 shRNA On Invasion. The presence of EP4 shRNA in SUM149/Clone 1 abolished 95% of invasion.
Figure 2D. Effect Of Sulprostone On Invasion. Sulprostone inhibited invasion by SUM149 cells in a dose dependent manner starting at 0.1 μM, with no effect of this agent on invasion by MDA-MB-231 cells.