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. Author manuscript; available in PMC: 2011 May 15.
Published in final edited form as: Cancer Res. 2010 May 11;70(10):4092–4101. doi: 10.1158/0008-5472.CAN-09-3834

Figure 1.

Figure 1

Activation of ER-extranuclear signaling promotes actin reorganization. A, MCF7 control or MCF7-PELP1-shRNA cells were lysed and expression of PELP1 was analyzed by Western blotting. B, MCF7 vector control and MCF7-PELP1-shRNA cells were cultured in 5% DCC serum containing medium treated with or without E2. The activation of signaling pathways was analyzed by Western blotting of total protein lysates with phospho-specific antibodies. Densitometric analysis of the western blots of phospho bands from triplicate samples were performed and corrected with the values of respective total bands. Each column is an average of triple determinations. Bars, SEM. *, p<0.05. **, P<0.001. C, MCF7 cells were treated with FITC-labeled EDC for 45 min and localization of EDC was analyzed by confocal microscopy (left panel). MCF7 and MCF7-PELP1-shRNA cells were treated with EDC and activation of signaling pathways was analyzed by Western blotting (C, middle panel). Quantitation of the bands was as described in Fig. B legend (C, right panel). D, MCF7 or MCF7-PELP1-shRNA cells were treated either with E2 or EDC and the F-actin status was analyzed by phalloidin staining and visualized by confocal microscopy.