Figure 6. Differential binding of OCH^HIGH and OCH^LOW iNKT clone derived TCR tetramers to endogenous lipid presenting CD1d molecules.

PE-conjugated recombinant iNKT TCR tetramers derived from OCH^HIGH (4C1369; red lines) and OCH^LOW (4C12; blue lines) iNKT clones, at increasing concentrations, were used to stain T2-CD1d lymphoblasts. Clear staining of vehicle-pulsed T2-CD1d (unfilled markers) was only seen with the OCH^HIGH TCR tetramer, whereas both iNKT TCR tetramers strongly bound to K7-pulsed T2-CD1d (filled markers). The black bar shows background staining of T2- cells with iNKT TCR tetramers.