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Primers used for PCR amplification of ORF2--ORF5 and NSP2 from PRRSV
| Genes | Primer sequence |
|---|---|
| ORF2 | 5'-ACGAAGCTTATGAAATGGGGTCTATG-3' |
| 5'-TATCTCGAGTCACCGTGAGTTCAAAAG-3' | |
| ORF3 | 5'-TTCATGATTTTCAGCAATGGCTAA-3' |
| 5'-GATGGTGATGTACACGGGCGT-3' | |
| ORF4 | 5'-ACGGCGGCAATTGGTTTCACCTA-3' |
| 5'-CGTGGTCAAGCATTTCCCCAACATA-3' | |
| ORF5 | 5'-CCTGAGACCATGAGGTGGGG-3' |
| 5'-TTTAGGGCATATATCATCACTGG-3' | |
| NSP2 | 5'-TGAYTGGRATGTTGTGCTYCCTGG-3' |
| 5'-ATGCGAGARAGCCAYTCCTGCGT-3' |
