Table 2.
Antibodies used in the study
| Antibody | Immunogen | Source | Host species | Isotype, Dilution used |
|---|---|---|---|---|
| anti-DIG | digoxigenin conjugated with KLH | Immunology Consultants Laboratory, CDIG-65A | chicken | polyclonal IgY, 1:500 |
| anti-Htt | human huntingtin protein a.a. 181-810 | Millipore, MAB2166 | mouse | monoclonal, IgG1κ, clone 1HU-4C8, 1:500 |
| anti-Ago2 | human Ago2 peptide KLMRSASFNTDPYVRE | Gift of Ramin Shiekhattar, The Wistar Institute | rabbit | polyclonal, 1:1000 |
| anti-dynein HC | rat dynein heavy chain a.a. 4320-4644 | Santa Cruz Biotechnology, sc-9115 | rabbit | polyclonal, IgG, 1:300 |
| anti-CPEB1 | mouse CPEB1 peptide SMEGLRHHSPLMRNQKN | Gift of David Wells, Yale University | rabbit | polyclonal, IgG, 1:1000 |
| Alexa Fluor® 488 goat anti-mouse IgG | mouse IgG (H + L) | Invitrogen, A-11001 | goat | polyclonal, 1:500 |
| Cy3 goat anti-mouse IgG | mouse IgG (H + L) | Jackson ImmunoResearch, 115-166-003 | goat | polyclonal, F(ab')2 fragment, 1:500 |
| DyLight 549 goat anti-chicken IgY (IgG) | chicken IgY (IgG) (H + L) | Jackson ImmunoResearch, 103-505-155 | goat | polyclonal, 1:500 |
| DyLight 649 goat anti-rabbit IgG | rabbit IgG (H + L) | Jackson ImmunoResearch, 111-495-144 | goat | polyclonal, 1:500 |
Abbreviations: DIG, digoxigenin; KLH, keyhole limpet hemocyanin; Htt, huntingtin; Ago, Argonaute; CPEB, cytoplasmic polyadenylation element binding protein