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. 2010 Jan 12;18(5):1035–1045. doi: 10.1038/mt.2009.293

Figure 4.

Figure 4

VIPase catalytic antibody partially reverses the characteristics of LentiVIP-DC. LentiVIP-DC were grown in the presence of daily added 180 µg of VIPase or control antibody (UPC10 IgG2a) as described in Materials and Methods. (a) LentiVIP-DC treated with or without VIPase were stained with anti-CD11c, or treated for 24 hours with lipopolysaccharide (LPS) and stained for CD40 expression. (b) Results are expressed as geometric mean of fluorescence (GMEAN) ± SD of three experiments performed in duplicate. Degree of significance P < 0.05. (c) TNF-α levels were determined by enzyme-linked immunosorbent assay in supernatants of LPS-treated cells. Three experiments were performed in duplicate. Degree of significance P < 0.05.