Figure 7.
LentiVIP-DC increases survival rate in the cecal ligation and puncture model. Male BALB/c mice (6–8 weeks old; 6/group) were subjected to cecal ligation and puncture as described in Materials and Methods. Immediately after the surgical procedure LentiVIP-DC and LentiGFP-DC (2 × 106 cells/animal) were injected intraperitoneal and 20 hours after the surgical procedure peritoneal exudate cells and fluid were collected. (a) LentiGFP-DC cells were detected by flow cytometry in the peritoneal cavity. (b) LentiVIP was detected by quantitative PCR after DNA extraction from peritoneal exudate cells. Primers to detect LentiVIP were used; LentiGFP-DC was used as negative control. (c) Peritoneal fluid was analyzed by enzyme-linked immunosorbent assay for cytokine production. Degree of significance P < 0.05. (d) The therapeutic effect of LentiVIP-DC was evaluated by survival rate over time. GFP, green fluorescent protein; IL, interleukin; MIP, macrophage inflammatory protein; TNF, tumor necrosis factor.