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. 2010 Mar 16;18(5):888–895. doi: 10.1038/mt.2010.44

Figure 4.

Figure 4

Vaccinia virus enhances vesicular stomatitis virus expression into human ex vivo tumor. (a) Human ex vivo rectal cancer and normal tissue, colon cancer metastases in liver and normal liver, brain and endometrial cancer tissues were inoculated with 1 × 108 pfu of VVDD-eGFP with or not VSVΔ51-DsRed. Enhanced green fluorescent protein (eGFP) and DsRed expression was monitored by fluorescence microscopy 48 hours after inoculation. (b) Summary of ex vivo tumor tissue sample. VSVΔ51 virus titers were determined to quantify viral replication. Each line corresponds to one patient [type of tumor and number of patient (#)]. Black and white bars: VSVΔ51-fold enhancement in doubly with VVDD (44 patients) and VVΔB18R (30 patients) compared to singly infected cultures. Combination VV/VSVΔ51 samples were infected by VVDD (1 × 107 pfu) and/or VSVΔ51 (1 × 108 pfu). Samples were processed for titration 48 hours after inoculation. Data were classified from the highest increase to the lowest one for VVDD/VSV combination. *** Not available data. A paired t-test stastistic comparing VVDD/VSV to VVDB18R/VSV. Samples tested for both VVDD/VSV and VVΔB18R/VSV were included for the statistic test. P < 0.05.