Figure 5.

VSVΔ51FAST enhances VVDD growth. (a) 786-O were left untreated or treated with VVDD-eGFP (0.1 MOI) for 2 hours and VSVFAST-DsRed (0.1 MOI) infection was then performed or not for 45 minutes. Enhanced green fluorescent protein (eGFP) (VV) and DsRed (VSVFAST) fluorescence were detected 48 hours by microscopy after VSVFAST infection. One of three independent experiment. (b) VVDD and VSVFAST titers 48 hours after infection. Mean ± SEM from three independent experiment. (c) Patient colon tumor specimen was infected by VVDD (eGFP) (1 × 10⁷ pfu) and/or VSVFAST (DsRed) (1 × 10⁸ pfu). eGFP and DsRed expression was monitored 48 hours after viral inoculation. (d) 72 hours after VSVFAST infection, viral oncolytic effect was assessed by a crystal violet assay. One of two independent experiments.