Figure 2.

Cellular uptake of proteins mediated by Pt-Dd. (a) HeLa cells were either untreated (left panel) or incubated for 1 hour with WW-OVA/Pt-Dd protein complexes (right panel). Protein internalization was detected by immunocytochemistry on fixed cells, colocalization of Pt-Dd, and WW-OVA is shown in the merge panel, with nuclei counter stained with Hoechst dye. (b) Mouse bone marrow-derived DCs were incubated for 3 hours with either Alexa 647-labeled WW protein only (gray histogram) or in complex with Pt-Dd (black histogram). Cells were trypsinized to remove surface bound proteins and protein internalization analyzed by flow cytometry. DC, dendritic cell; OVA, ovalbumin; Pt-Dd, penton-dodecahedron.