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. 2010 Feb 23;18(5):1046–1053. doi: 10.1038/mt.2010.16

Figure 3.

Figure 3

Vaccination with WW-OVA/Pt-Dd generates OVA-specific CD8+ T-cell responses. C57BL/6 naive mice (N = 3) were immunized twice with WW-OVA/Pt-Dd protein complexes or PBS. CD8+ T-cell responses were measured 14 days postinjection on splenocytes. (a) H-2Kb-OVA (SIINFEKL) tetramer staining on CD8+ cells from mice injected with PBS (left panel) or Pt-Dd/WW-OVA (right panel). (b) Specific killing of B16-OVA target cells by FACS-based cytotoxicity assay. A quantitative analysis of viable target cells was performed using FITC-labeled calibration beads and the percentage of specific lysis was calculated with the formula [1 − (R1:1/R1:A)] × 100, as described in Supplementary Materials and Methods. FACS, fluorescence-activated cell sorting; FITC, fluorescein isothiocyanate; OVA, ovalbumin; PBS, phosphate-buffered saline; Pt-Dd, penton-dodecahedron.