Figure 2.

Hematoxylin and eosin staining of muscle cross sections. The tibialis anterior muscles of mdx dystrophic mice were injected with (a) phosphate-buffered saline or (b) an AAV-1 carrying the sh-AcvRIIb-3. One month after injection, mice were killed, and the muscles were mounted in OCT and then frozen. Transverse 8 µm frozen sections were performed using a cryostat, and sections were stained with hematoxylin and eosin.