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. 2010 Mar 15;115(23):4853–4861. doi: 10.1182/blood-2009-07-235093

Figure 3.

Figure 3

Hipk knockdown causes cell accumulation in S phase and increased apoptosis. Ter119-negative fetal liver cells infected with shRNA-containing retroviruses were cultured in Epo-containing media. Aliquots of cells were removed at 24, 36, and 48 hours and stained with propidium iodide for FACS analysis of cell cycle, or annexin V and 7-AAD for FACS analysis of apoptosis. (A-B) Cell cycle: percentage of cells from each infection in S phase (A) or G1 (B); n = 3 (mean ± 2 SEM). *P < .05. **P < .01. (C-D) Apoptosis: percentage of cells that are actively undergoing apoptosis but not dead (C; 7-AAD-negative, annexin V-positive) or dead cells (D; 7-AAD, annexin V–double-positive); n = 3 (mean ± 2 SEM). *P < .05.