Table 1.
mRNA expression changes in Hipk2-depleted fetal liver cultures as determined by quantitative PCR analysis
| RefSeq ID | Gene symbol | Gene name | Fold change | P | Description |
|---|---|---|---|---|---|
| Erythropoiesis | |||||
| Down-regulation | |||||
| NM_031158 | Ank1 | Ankyrin 1, erythroid | −0.77 | < .009 | Actin cytoskeleton organization |
| NM_019670 | Diap3 | Diaphenous homolog 3 (MDia2) | −0.50 | < .112 | Actin binding |
| NM_183428 | Ebp4.1 | Band 4.1 | −0.84 | < .001 | Actin cytoskeleton organization |
| NM_133245 | Edrf1 | α-Hemoglobin stabilizing protein | −0.59 | > .011 | Hemoglobin binding |
| NM_010149 | EpoR | Erythropoietin receptor | −0.57 | < .013 | Cytokine receptor activity |
| NM_008089 | Gata1 | GATA-binding protein 1 | −0.50 | < .001 | Regulation of transcription |
| NM_008090 | Gata2 | GATA-binding protein 2 | −0.36 | < .323 | Regulation of transcription |
| NM_010369 | Gypa1 | Glycophorin A | −0.55 | .084 | Cytoskeletal anchoring protein |
| NM_010635 | Klf1 | Kruppel-like factor 1, erythroid (EKLF) | −0.46 | < .001 | Regulation of transcription |
| NM_009007 | Rac1 | RAS-related C3 botulinum substrate 1 | −0.52 | < .066 | Small GTPase-mediated signal transduction |
| NM_009008 | Rac2 | RAS-related C3 botulinum substrate 2 | −0.36 | > .002 | Small GTPase-mediated signal transduction |
| NM_011403 | Slc4a1 | Band 3 | −0.81 | < .008 | Anion exchange; cytoskeleton |
| NM_013675 | Spnb1 | Spectrin 1, β | −0.61 | < .017 | Actin cytoskeleton organization |
| NM_011638 | Trfr2 | Transferrin receptor 2 | −0.10 | > .150 | Iron ion transport activity |
| NM_009569 | Zfpm1 | Friend-of-GATA1 (FOG1) | −0.56 | < .004 | Regulation of transcription |
| Up-regulation | |||||
| NM_019740 | Foxo3 | Forkhead box O3a (Foxo3a) | 0.04 | > .019 | Regulation of transcription |
| Heme biosynthesis and hemoglobin production | |||||
| Down-regulation | |||||
| NM_009653 | Alas2 | ALAS2 | −0.50 | .003 | Heme biosynthesis |
| NM_007998 | Fech | Ferrochelatase (Fech) | −0.16 | < .001 | Heme biosynthesis |
| NM_008218 | Hba-a1 | Hemoglobin-α adult chain 1 | −0.64 | > .039 | Heme binding, oxygen transport |
| NM_010405 | Hba-x | Hemoglobin X, α-like embryonic chain | −0.29 | < .074 | Heme binding, oxygen transport |
| NM_008220 | Hbb-b1 | Hemoglobin, β adult major chain | −0.57 | > .003 | Heme binding, oxygen transport |
| NM_008219 | Hbb-bh1 | Hemoglobin Z, β-like embryonic chain | −0.60 | > .012 | Heme binding, oxygen transport |
| NM_026331 | Slc25a37 | Mitoferrin 1 | −0.83 | < .011 | Mitochondrial iron ion transport |
| Cell cycle and proliferation | |||||
| Down-regulation | |||||
| NM_009743 | Bcl2l1 | Bcl-2 like 1 (Bcl-XL) | −0.61 | < .007 | Regulation of apoptosis |
| NM_009829 | Ccnd2 | Cyclin D2 | −0.58 | < .054 | Cell cycle control at G1/S transition |
| NM_007669 | Cdkn1a | Cyclin-dept kinase inhibitor 1a (p21, cip1) | −0.33 | < .001 | Cell cycle progression through G1 |
| NM_009875 | Cdkn1b | Cyclin-dept kinase inhibitor 1b (p27, kip1) | −0.56 | .004 | Cell cycle control at G1 |
| NM_010786 | Mdm2 | Murine double minute 2 | −0.82 | Negative regulator of p53 | |
| NM_009029 | Rb1 | Retinoblastoma 1 | −0.70 | .000 | Tumor suppressor, regulation of cell cycle |
| NM_011640 | Trp53 | p53 (transformation related protein 53) | −0.38 | .144 | Tumor suppressor, cell division regulation |
| Up-regulation | |||||
| NM_007628 | Ccna1 | Cyclin A1 | 0.33 | .339 | Cell cycle regulation |
| NM_007631 | Ccnd1 | Cyclin D1 | 0.13 | < .093 | Reentry into mitotic cell cycle |
| NM_001037134 | Ccne2 | Cyclin E2 | 1.31 | > .021 | Cell cycle regulation |
| NM_007658 | Cdc25a | Cell division cycle 25 homolog A | 0.18 | < .068 | Cell cycle regulation |
| NM_010848 | Myb | Myb proto-oncogene (C-myb) | 0.59 | > .040 | Transcription; cell cycle control |
| NM_007483 | RhoB | Ras homolog gene family, member B | 1.91 | < .081 | Negative regulation of cell cycle |
| Miscellaneous | |||||
| Down-regulation | |||||
| NM_007913 | Egr1 | Early growth response 1 | −0.06 | < .037 | Regulation of transcription |
| NM_001033228 | Itga1 | Integrin-α1 precursor (VLA-1; CD49a) | −0.68 | > .003 | Cell adhesion |
| NM_023045 | Xpo7 | Ran-binding protein 16 (Exportin 7) | −0.74 | > .007 | Protein export from nucleus |
| NM_010019 | Dapk | Death-associated protein kinase 2 | −0.58 | .000 | Positive regulator of apoptosis |
| Up-regulation | |||||
| NM_013768 | Prmt5 | Protein arginine N-methyltransferase 5 | 0.94 | < .799 | Methyltransferase activity |