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. Author manuscript; available in PMC: 2010 Jun 23.
Published in final edited form as: Cell Cycle. 2007 Sep 28;7(1):81–88. doi: 10.4161/cc.7.1.5103

Figure 2.

Figure 2

NCX-4016-induced apoptosis in CR ovarian cancer cells. Cells were treated with NCX-4016 (100 μM) for 48 h and the sub-G1 population for 20,000 events within a fixed gate was analyzed. Representative flow-cytometry profile for (A) control (vehicle-treated) and (B) NCX-4016-treated groups. (C) Immunoblot analysis of apoptotic proteins. Cleavages in caspase-9, caspase-8, caspase-3, caspase-7, cytochorome c, and PARP are shown in cells treated with NCX-4016 (100 μM) for 24 or 48 h. (D) Intensity of cytochrome c band as quantified by densitometry. (E) Inhibition of p53 by pifithrin did not reverse the NCX-4016 induced apoptosis. The CR cancer cells were pretreated with the pifithrin 20 μM for 1 h and then treated with NCX-4016 for 48 h. The cells were analyzed for cleaved caspase-9, caspases-7 and PARP by immunoblot assay.