Effect of NCX-4016 on Bcl-2 family protein expressions in CR ovarian cancer cells. Cells were treated with NCX-4016 (100 μM) for 24 or 48 h and analyzed for various Bcl-2 family proteins. (A) Representative Western blot images showing time-dependent expressions of Bax, Bak, Bcl-2, Bcl-XL, and actin. Intensity of Bax (B) and Bcl-2 (C) as quantified by densitometry are also shown. Data represent mean ± SE of three independent experiments. *p < 0.05 as compared to the Control group. The results reveal a significant contribution of the Bcl-2 proteins, particularly Bax/Bcl-2, to the induction of apoptosis in the CR cancer cells treated with NCX-4016.