Table 2. Thermodynamic Parameters for the Binding of Histamine to rRaHBP2(D24R) and Wild-Type rRaHBP2 in PBS at pH 7.4.
| temp (K) | ΔH° (kJ/mol) | TΔS° (kJ/mol) | ΔG° (kJ/mol) | Kd (nM) | |
|---|---|---|---|---|---|
| rRaHBP-2(D24R) | |||||
| 278 | −42.7a ± 0.9b | 6.5 ± 1 | −49.2 ± 0.6 | 2.4 ± 0.6 | |
| 288 | −48.3 ± 3.1 | 0.4 ± 4.5 | −48.8 ± 1.1 | 2.9 ± 1.3 | |
| 298 | −58.3 ± 1.2 | −9.3 ± 2.5 | −49.1 ± 1.4 | 2.5 ± 1.4 | |
| rRaHBP-2 (wt) | |||||
| H site | 298 | −70.9 ± 7.1 | −24.4 ± 7.3 | −46.5 ± 1.3 | 7.0 ± 3.6 |
| L site | 298 | −53.2 ± 3.3 | −12.0 ± 3.6 | −41.2 ± 1.5 | 59.6 ± 34.8 |
a
Values are expressed as the mean of three measurements.
b
Standard errors were determined from duplicate experiments by error propagation.