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. Author manuscript; available in PMC: 2011 Jun 29.
Published in final edited form as: Biochemistry. 2010 Jun 29;49(25):5321–5330. doi: 10.1021/bi100434c

FIGURE 2.

FIGURE 2

Self-cleavage of human CPEB3 WT ribozyme analyzed on a native gel. (A) Self-cleavage reaction of −8/68 fractionated on 10% native gel (Lanes 1 – 8). RNA was 5′-end labeled with [γ-32P]GTP during transcription and reactions were initiated by addition of 10 mM MgCl2. Full-length RNA partitions into precursor R1 and R2 bands, and upon self-cleavage, a −8/−1 product, P. Marker bands corresponding to native an non-native RNAs that were previously purified on a qualitative native gel, renatured at 50°C and fractionated on this native gel are shown in Lanes 9 and 10 respectively. (B) Time-dependent change in the three RNA species fractionated on native gels. Inset is a plot of fcleaved versus time for the data in panel A, where P/(R1+R2+P) represents the cleaved fraction. Each data point is the average of at least two trials ± the standard error of the experiments. Data were fit to eq 1.