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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Biochim Biophys Acta. 2010 May 5;1798(8):1480–1484. doi: 10.1016/j.bbamem.2009.11.023

Figure 4.

Figure 4

Left: Representative current traces of OmpA wild-type and mutant proteins isolated from cytoplasmic inclusion bodies of E. coli BL21(DE3)pLysS cells, purified by Ni agarose chromatography, reconstituted into C8E4 micelles, and incorporated into planar lipid bilayers of DPhPC at room temperature between aqueous solutions of 1M KCl in 20 mM Hepes, pH 7.4. Bar at right of the traces indicates the closed state of the channel. Clamping potential was +100 mV. Right: Histograms taken from records of ~ 10 minutes at room temperature.