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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Chem Biol Drug Des. 2010 Mar 30;75(6):551–562. doi: 10.1111/j.1747-0285.2010.00974.x

Figure 4.

Figure 4

Loss of inhibitory potency when CPNGRC is incubated at elevated temperatures and pH. CPNGRC was incubated at 37°C and pH 8.5 for 2 hours (□) and then 24 hours (◁). The resultant peptide was assayed for its ability to inhibit APN catalyzed hydrolysis of pLeuNA, relative to peptide kept at pH 7.5 and at 4°C (◇). After 24 hours, the peptide shows almost no inhibition when compared to activity assays in the absence of peptide (○). The concentration of CPNGRC was 50 μM concentration in these assays.