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. 2010 May 17;107(22):10097–10102. doi: 10.1073/pnas.0914918107

Fig. 1.

Fig. 1.

Cellular and molecular characteristics of fibroblasts of HH1. (A) Cellular senescence. Results are expressed as the percentage of SA–β-galactosidase (Gal)-positive cells. Primary fibroblasts are from a healthy control (Ctl; n = 217; black, passage 11), a dyskerin-deficient patient (n = 234; gray, passage 4), and HH1 (n = 219; white, passage 6). (B) Colocalization of 53BP1 foci with TRF2 representing TIFs can be detected in cells of HH1. (C) Quantification of TIFs. The fraction of fibroblasts from a control (Ctl; n = 204) and HH1 (n = 89) presenting with TIFs and the number of TIFs per TIF-positive cell were quantified. (D) Mean telomere length of primary fibroblasts from a healthy control (Ctl2), a dyskerin-deficient patient (Dkn), HH1, and control DNA from the TRF-kit (Ctl1; Roche) was estimated by the TRF method. All the results in this figure were obtained with cells having the passage number indicated in A.