Fig. 2.

Surgically induced OA is more severe in Sulf^−/− mice. (A) Surgical OA was induced in WT and Sulf^−/− mice at the age of 8 weeks (n = 8–10 mice per group) by resecting the medial meniscotibial ligament and harvesting the knee joints 4 weeks after surgery for safranin O staining. Arrow indicates roughening of the articular surface. (B) Sulf^−/− mice showed significantly increased Mankin and summed scores compared with WT after surgery. ^†, P < 0.01. (Scale bars: low magnification, 200 μm; high magnification, 50 μm.)