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Fitted SecA-SecYEG binding parameters from ATPase binding curves presented in Fig. 4B
| Kd (μM) | kcat (min-1) | |
| Delipidated SecYEG (-CL) | 2.86 ± 1.08 | 16.4 ± 1.5 |
| Supplemented SecYEG (+CL) | 0.42 ± 0.25 | 26.30 ± 3.8 |
| Delipidated SecYYE2G2 (−CL) | 0.41 ± 0.12 | 15.7 ± 1.1 |
| Supplemented SecYYE2G2 (+CL) | 0.34 ± 0.13 | 30.6 ± 2.4 |
Data were fitted according to Eq. S2. ± indicates SD; n≥3.
