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. 2010 May 17;107(22):10226–10231. doi: 10.1073/pnas.0913065107

Fig. 2.

Fig. 2.

Impact of mutating the 3′ terminal nucleotide of the template on RSV RNA replication. (A) Quantitation of encapsidated template generated in transfections in which plasmid encoding L was present (+L) or absent (-L). (B) Quantitation of replication products generated from the mutant minireplicons. Black bars represent the replication product in the total RNA samples; white bars represent replication product measured in the MCN-treated RNA samples. (C) Direct comparison of the amounts of total and MCN-resistant replication product generated in the WT and Δ1U reactions. Each RNA value was determined based on Northern blot analysis, such as that shown in Fig. S1. Levels were calculated relative to the respective WT value for A and B, or relative to the WT or Δ1U total RNA values for C (set at 1.0 in each case). Each error bar represents the standard error of the mean from at least three independent experiments.