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. 2010 May 17;107(22):10038–10043. doi: 10.1073/pnas.0911716107

Fig. 3.

Fig. 3.

The C-terminus of Mdm2 associates with the proteasome. (A) Bacterially expressed V5-tagged N-terminus (aa 1–200), V5-tagged central domain (aa 200–299) or V5-tagged C-terminal domain (aa 300–491) of Mdm2 were mixed with bacterially expressed and purified GST, S6b fused to GST or S8 fused to GST. GST was pulled down and associated Mdm2 was determined by Western blotting. (B) Schematic drawing of the C-terminal deletion mutants of Mdm2. Bacterially expressed V5-tagged C-terminal domain of Mdm2 and the indicated deletion mutants were mixed with bacterially expressed and purified GST or S6b fused to GST. GST was pulled down and associated C-terminal fragments of Mdm2 were determined by Western blotting. (C) Bacterially expressed C-terminal domain of Mdm2 harboring the indicated mutations were mixed with bacterially expressed and purified GST or S6b fused to GST. GST was pulled down and associated Mdm2 was determined by Western blotting.