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. 2010 May 13;107(22):10166–10171. doi: 10.1073/pnas.0909112107

Fig. 3.

Fig. 3.

The SLP-76 4KE mutant disrupts GADS microcluster formation. Confocal images of GADS-mCherry and SLP-76-EYFP cluster formation and distribution at the interface of transfected cells and anti-CD3–coated slides (A). Jurkat cells were cotransfected with and GADS-mCherry (red) and SLP-76-EYFP WT (green) (Upper) or with GADS-mCherry and SLP-76-EYFP 4KE mutant (Lower). (Scale bars, 10 μm.) Insets show time-lapse series of GADS and SLP-76 WT microcluster movement in selected regions imaged over 50 s. Histogram shows the speed (μm/s) traveled by individual clusters over time within cells cotransfected with SLP-76-EYFP WT and GADS-mCherry (B).