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. 2010 May 17;107(22):10296–10301. doi: 10.1073/pnas.1004788107

Fig. 1.

Fig. 1.

Gene expression and zinc transport of the Arabidopsis ZIP4 zinc transporter. (A) Growth of zrt1zrt2 S. cerevisiae cells carrying either the empty vector (zrt1zrt2 Ø) or expressing ZIP4 (zrt1zrt2 ZIP4) was assayed by spotting serial dilutions of cells (OD600 is shown on the left) on SD-URA selective medium with 0.4 or 0.8 mM ZnCl2. (B) OD measurements at the indicated time-intervals of zrt1zrt2 Ø and zrt1zrt2 ZIP4 on SD-URA selective liquid medium with 0.4 mM ZnCl2 (mean ± SEM). (C) Relative transcript levels (RTL) of ZIP4 in 3-week-old Arabidopsis seedlings grown in MS medium, with 30 μM ZnSO4 (Zn+) or without (Zn-) (mean ± SEM). (D) GUS expression analysis in roots of Arabidopsis 3-week-old seedlings, stably transformed with a ZIP4promoter::GUS construct, grown in MS medium, with 30 μM ZnSO4 (Zn+) or without (Zn-).