Fig. 3.
CFIm25 specifically recognizes two UGUA elements. (A) Bar graph representation of the electrophoretic mobility shift assay (EMSA) data of CFIm25 variants binding to a 21 nt PAPOLA poly(A) site RNA containing two UGUA elements. (B) EMSA data of wild type CFIm25ΔN21 binding to various RNA sequence variants. A single prime represents the mutation on the first UGUA element, and double prime represents the mutation on the second UGUA element. Experiments were done in triplicate and all the bound fractions were plotted relative to CFIm25ΔN21 and the wild type PAPOLA RNA. The error bars represent the standard deviation.